Mouse Anti-Human α-Heavy Chain Monoclonal Antibody (mAb0300) by Nanjing Miaodi Biotechnology

Our Mouse Anti-Human α-Heavy Chain Monoclonal Antibody (mAb0300) is a highly specific and reliable reagent designed for the precise detection of human immunoglobulin α-heavy chains (IgA). Engineered using advanced hybridoma technology by immunizing mice with purified human IgA immunoglobulin (derived from human colostrum), this monoclonal antibody ensures exceptional consistency, sensitivity, and minimal cross-reactivity. It binds only to the heavy chain of human IgA antibody, and has no cross-linking with human IgG antibodies (γ1-4), IgM, or IgE, and does not bind to the light chain (Fig. 1, 2).

The mAb0300 is ideal for applications such as ELISA, Western blotting, immunohistochemistry, and flow cytometry. It provides accurate and reproducible results across a wide range of research and diagnostic settings. Each batch is rigorously validated to meet high-quality standards, making it a trusted choice for immunology and biomedical research.

Fig. 1 Cross-reactivity of MAb0300 with human IgM, IgG, and IgE.

Fig. 2 mAb0300 binds only to the heavy chain of human IgA antibody and not to the light chain.

In the human IgA indirect ELISA system, this monoclonal antibody, as an enzyme-labeled secondary antibody (Fig. 10), exhibits advantages such as low background and high signal-to-noise ratio, making it suitable for the determination of human IgA antibody titers after vaccination and during the acute phase of infectious diseases.

Fig. 3 Schematic diagram of indirect IgA ELISA for 10 individuals.

Mouse anti-human α-heavy chain monoclonal antibodies (mAb0300, HRP0030)